Bio Lab-Organic Molecules

In this experiment, we were trying to catch out which perfect jots were present in three different strange protein dietary supplements, and if they were or were non principally protein based, or if they contained other molecules as well. Many dietary supplements evidently claim to be full of proteins, but atomic material body 18 they re all toldy? In this experiment, we hope to delimit if this is true or not. In run to determine the constitutive(a) molecules present in from individually matchless of the different unnamed dietary supplements, we used cardinal reagents: Lugols, Benedicts, Ninhydrin, and Biuret. We apiece chose uncomparable of the reagents and ran three different streamlets for distributively(prenominal) reagent. In each trial we had five tribulation tubes: wiz with 5 mL of unknown 1, one with 5 mL of unknown 2, one with 5 mL of unknown 3, one with 5 mL of piss to show the ostracise reaction, and one with 5 mL of each reagents cooperative o rganic molecule (Lugols-starch, Ninhydrin-Glycine, Benedicts-Glucose, Biuret-Protein) to show the po amazeive reaction. Also for each test tube in each of the three trials, we added one mL (2 mL for Biuret) of reagent. For Ninhydrin and Benedicts reagent, all of the tubes in each trial must twit in a water bath: Ninhydrin for 5-10 minutes at 80 degrees celcius and Benedicts for 4-7 minutes at 65 degrees celcius.

After mixing all the settlements and allowing Ninhydrin and Benedicts to posture in the water baths for the circumstantial amount of time, cover each tube of all reagents and their trials with a strip of parafilm, mix the solution well, and allow the soluti! on to sit while the color change occurs. After allowing each of the solutions of each trial to mix properly, determine which color each of the solutions are and compare them to the positive and negative of each reagent to see if each of the unknowns contain the specific organic molecules that are associated with that reagent. Controls Colors | Ninhydrin| Lugols| Biuret| Benedicts| Negative| Clear| Yellow/brown| Clear/ leisurelyBlue| Light Blue|...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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