Introduction to Microbiology (CP4089) - Experiment 4: Colony Count Methods: Viable Cell Number of Commercial Active Dried Yeasts

Summary This purpose of this investigate was for students to do the liquidation figuring methods, estimating the practicable cell event of commercial active voice desiccate yeasts (ADY). This prove allowed the students to per run the headquarters moot technique by resultant dilution and two common methods, scattering home office and snap plate to specialise the dependance forming unit (CFU) of yeasts A ten-fold dilution is mapping of goods and servicesd in this experiment, the try on distribution is diluted until it reached the 10-9 dilution. Plating for disseminate plate started from 10-5, 10-6, 10-7 and 10-8 dilution while for pour plate, it started from 10-6, 10-7, 10-8 and 10-9 dilution. Having incubated inverted at mode temperature (25oC) for 2 days, the practicable cells for spread plate and pour plate were calculated. The CFU/g obtained for spread plate and pour plate were 2.2 X 1010 CFU/g and 3.7 X 1010.the CFU/g respectively. Introduction Colony count methodology is iodin of the most accurate methods to determine the figuring of the organisms in a given sample. It enumerates the number of certain live, possible cells in the sample that form colonies on a desirable agar medium. As the optimum medium and conditions varies for one sample to another, the colony count methods provide an estimate of the number of viable cells according to the medium employed, time and temperature of incubation. Each colony that appears on the agar plate arising either from a flock of cells or from a single cell is referred as a colony forming unit (CFU). The sample used in this experiment is active dried yeasts (ADY).

A serial dilution is performed to suspend the yeasts containing ingathering in water so that the number of microorganisms per ml is low-spirited becoming to be counted, when the sample is plated. Cells in overcrowded plates may not form colonies but may... Hi, Its regarding the results and calculation part. Theres some mistakes I think. For run the cfu/g for the spread plate and pour plate, the dilution number should use the same. Which mode that both should use 10^-8, whr ^ means to the causation of. whence if use the correct dilution number to count the cfu/g, the spread plates cfu is high than the pour plates cfu, which tally with the theoretical CFU. If you want to ascertain a full essay, order it on our website: OrderCustomPaper.com

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